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Pros and cons

Multiplication rates vary hugely between genera due to different plant structures and growth rates. Some plants cannot be propagated in tissue culture at all, and therefore have a much longer waiting period between discovery and introduction to the market.

 

Although tissue culture is a very desirable propagation method due to its speed, there are many stages at which plants may fail to adapt to the next phase, so it is far from 100% reliable. On the other hand, the tissue culture process often causes plants to mutate, and many exciting new varieties have been created in this way.

 

Tissue culture doesn't come cheap, and the price you pay for a plant can reflect this. But would you prefer to wait another 3 years?

Hybridiser
 
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Tissue culture
07-02-2004 20:36 It may be an unfamilliar term to gardening enthusiasts, but to the professional perennial industry, tissue culture is the buzz-word if you want to reproduce plants quickly.
By Miriam Young   

Tissue culture has revolutionised the plant industry. It enables as many as 5,000 copies of a unique new plant to be produced in within a year, while other propagation methods can take 3 times as long.

 

Propagation by tissue culture is an in vitro, asexual method used to produce large quantities of identical plant copies. It is a very specialised method carried out under sterile conditions in a laboratory, often involving microscopic procedures.

 

 

The first stage is to take the cuttings. A plant’s entire genetic information is contained in specific areas that produce new shoots. These parts are cut out of the plant and placed in a gel that contains hormones and nutrients that induce new shoot growth.

 

 

 

As the tiny cuttings grow into tiny, but complete plants with new shoots of their own, they can also be used to take cuttings from. This cycle can take as little as a few weeks, meaning that it is possible to produce as many as 5,000 copies from a single plant in one year.

 

 

When the required number of copy plants have been produced, the next stage is to get them to grow roots, and this is done by transferring them to a gel with different hormones that induce root growth.

 

 

Once the plants have sufficient roots, they can then be planted in soil for the first time, usually into cells just a couple of centimeters across. They are still extremely small and delicate at this time and need to be nurtured very carefully as they adapt from the sterile laboratory conditions to their new surroundings.

 

Look but don't touch! Gert van Eijk-Bos gives us a sneaky peak at new plants growing under a protective moisture blanket.

 

As the roots start to establish themselves in the soil, the plants can be transplanted into larger cells. This is the point at which they are often passed onto more traditional growers to take over.

 
Biography
Credit for the back-room boy
By Miriam Young
It’s a big claim to rest on one man’s shoulders, but Gert van Eijk-Bos changed the face of gardening.
The Nursery
Revolutionising the Perennial World
By Miriam Young
Gert van Eijk-Bos's plant discoveries are made at the Vitro Westland tissue culture laboratory.
Other varieties
More from Gert
By Miriam Young
Gert van Eijk-Bos is also the man behind a number of perennial varieties you may well recognise.
Technical information
Tissue culture
By Miriam Young
It may be an unfamilliar term to gardening enthusiasts, but to the professional perennial industry, tissue culture is the buzz-word if you want to reproduce plants quickly.